TRYPTOSE (BACTERIOLOGICAL GRADE)

Description

SR. No	Test Parameter		Specification	Reference
1.0	Description		Creamy yellow coloured spray dried powder.	In-house
2.0	Colour		Creamy yellow coloured spray dried powder.	In-house
3.0	Solubility		Completely Soluble in water.	In-house
4.0	Appearance of solution (2%w/v) before autoclaving		Solution is clear and light yellow.	In-house
5.0	Appearance of solution (2%w/v) after autoclaving		Solution is clear and light yellow.	In-house
6.0	pH of 2% solution		6.00 – 7.50	In-house
7.0	Phosphate buffer test Single and double strength		Solution is clear and light yellow without any precipitate.	In-house
8.0	Loss on drying (105⁰C)		NMT 7.00 %w/w.	In-house
9.0	Total Nitrogen		NLT 12.00 %w/w.	In-house
10.0	Alpha amino nitrogen		NLT 2.50 %w/w.	In-house
11.0	Residue on Ignition		NMT 15.00 %w/w.	In-house
12.0	Microbial Content		Test passes.	In-house
13.0	Total aerobic microbial count		Not more than 5000 CFU /gm.	In-house
14.0	Total combined yeast and mould count		Not more than 500 CFU /gm.	In-house
15.0	Pathogen			In-house
15.1	E. coli.		To be absent.	In-house
15.2	S. typhi		To be absent.	In-house
15.3	S. aureus		To be absent.	In-house
15.4	P. aeruginosa		To be absent.	In-house
16.0	Growth promotion test			In-house
	Test organism	ATCC No.	Expected Result	–
16.1	Escherichia coli	25922	Good	In-house
16.2	Staphylococcus aureus	25923	Good	In-house
16.3	Salmonella typhimurium	14028	Good	In-house

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